Effect of C/EBPβ or C/EBPδ on granulopoiesis.
C/EBPβ or C/EBPδ induces granulopoiesis. (A) Total cellular proteins corresponding to 1 × 106 cells, from subclones transduced with pBabePuro-C/EBPβWT-ER (βWT-ER), pBabePuro-ER (ER), or pBabePuro-C/EBPδWT-ER (δWT-ER) or from parental 32D cl3 cells (32D) were subjected to Western blotting with the use of a human ER antiserum. The position of a truncated C/EBPβWT-ER isoform is indicated by an asterisk. (B) 32D-C/EBPβWT-ER-1 cells, 32D-C/EBPβWT-ER-2 cells, and 32D-ER cells were exposed to 1 μM estradiol for 0, 1, 2, or 3 days. Total RNAs prepared each day were subjected to Northern blotting for MPO, LF, PU.1, and β-actin by sequential probing of the same blot. (C) 32D-C/EBPδWT-ER-1 and 32D-C/EBPδWT-ER-2 cells were analyzed similarly.
