Effect of C/EBP inhibition on endogenous and exogenous G-CSFR expression.
Inhibition of C/EBPs reduces endogenous, but not exogenous, G-CSFR expression. (A) Total cellular RNAs were prepared from 32D–KαER-1 and 32D–KVER-2 cells proliferating in IL-3, after exposure to 4HT or the ethanol vehicle for 2 days. These RNAs were subjected to Northern blotting for G-CSFR and β-actin. (B) The 32D–KαER-1, 32D–KVER-2, 32D-KαER/GR, and 32D-KVER/GR cells were cultured with 4HT or the ethanol vehicle for 2 days. Then, 2 × 106 cells from each culture were incubated with biotin–G-CSF in the absence (thick lines) or presence (thin lines) of 1000-fold excess unlabeled G-CSF. The cells were then incubated with streptavidin-PE, fixed, and subjected to FACS analysis.
