Fig. 3.
Fig. 3. Reactivity of isolated scFv with factor V/factorVIII and human/porcine factor VIII hybrids. / Binding of scFv to recombinant factor V/VIII (A) and porcine/human factor VIII (B) hybrids was assessed by immunoprecipitation. (Lane 1) positive control (34, anti-A2 scFv VK34); (lanes 2-10) 5, 9, 14, 8, 10, 11, 13, 1, and 16: scFv corresponding to clones EL-5, EL-9, EL-14, WR8, WR10, WR11, WR13, WR1, and WR16; (lane 11) negative (−) control. On the right, the fragments used are indicated. Sequences corresponding to human factor VIII are depicted as hatched boxes. At the top of the figure, the 2 classes of VH gene segments that encode the different clones are given. Clone EL-5, EL-9, EL-14, WR8, WR10, WR11, and WR13 are derived from VH gene segments DP-10, DP-14, and DP-88, whereas clones WR1 and WR16 are derived from VH gene segment DP-5.

Reactivity of isolated scFv with factor V/factorVIII and human/porcine factor VIII hybrids.

Binding of scFv to recombinant factor V/VIII (A) and porcine/human factor VIII (B) hybrids was assessed by immunoprecipitation. (Lane 1) positive control (34, anti-A2 scFv VK34); (lanes 2-10) 5, 9, 14, 8, 10, 11, 13, 1, and 16: scFv corresponding to clones EL-5, EL-9, EL-14, WR8, WR10, WR11, WR13, WR1, and WR16; (lane 11) negative (−) control. On the right, the fragments used are indicated. Sequences corresponding to human factor VIII are depicted as hatched boxes. At the top of the figure, the 2 classes of VH gene segments that encode the different clones are given. Clone EL-5, EL-9, EL-14, WR8, WR10, WR11, and WR13 are derived from VH gene segments DP-10, DP-14, and DP-88, whereas clones WR1 and WR16 are derived from VH gene segment DP-5.

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