Quantification of vascular lesions induced by systemic CD95 engagement.

Wt mice were injected with JO2 (1) (n = 9), JO2 plus caspase-8 inhibitor (2) (n = 4), control immunoglobulin (3) (n = 4), LPS (> 150-fold excess of LPS content in JO2) (4) (n = 4), smCD95L (5) (n = 4), smCD95L plus caspase-8 inhibitor (6) (n = 4), sCD95L (7) (n = 5), the anti-FLAG antibody used to multimerize the sCD95L (8) (n = 5), or remained untreated (9) (n = 5); JO2 was injected in lpr mice (10) (n = 7), in gld mice (11) (n = 7), or in TNF-α^−/−–Ltα^−/−mice (12) (n = 4). Percentages are mean ± SD of 2 independent counts on tissue sections from liver, heart, kidneys, and brain of(n) different mice, as described in “Materials and methods.” *P < .007 compared to 1; °P < .03 compared to 5;⁺P < .002 compared to 1, 11, and 12 (Wilcoxon test).