Fig. 3.
Systemic allogeneic immune responses induce disseminated vascular endothelial cell death.
(A) The TUNEL assay shows apoptosis (brown staining) of vascular endothelial cells (filled arrowheads) and additional cell types in tissue sections from liver, heart, kidney, and brain of immunodeficient SCID recipient mice killed 2 days after transfer of 108lymphocytes from allogeneic wild type (WT) mice or from allogeneic CD95-defective lpr (LPR) mice, whereas no apoptotic lesions were detected in SCID recipient mice 2 days after transfer of 108 lymphocytes from allogeneic CD95L-defectivegld (GLD) mice (the unstained endothelial cells are indicated by open arrowheads). (B) Endocardial lesions (heart) and subarachnoidal hemorrhage suffusions (brain), as well as typical electron microscopy features of endothelial cell apoptosis (kidney) were observed in SCID recipients killed 2 days after transfer of 108 lymphocytes from allogeneic wt mice (filled arrowheads).