Cytofluorographic phenotype of PrMCs.

(A) Flow cytometric analysis of in vitro–derived PrMCs and MCs was carried out as previously detailed24 to assess FSC and SSC as well as surface expression of c-kit. By light scatter, the PrMCs are identified as R2 and MCs as R1. The heavy line in the c-kit histogram identifies the low SSC population (PrMCs) and the thin line identifies the IgG control mAb. (B) Surface expression of various Ag on column-purified CD14⁻ PrMCs. The mAbs used are identified in “Materials and methods.” The depicted results are representative of the 2 experiments performed.