PrMC adhesion to immobilized P-selectin, E-selectin, and VCAM-1 under defined flow conditions.
Coverslips containing human recombinant E-selectin, P-selectin, or VCAM-1 at saturating concentrations were prepared and PrMCs (5 × 105/mL in perfusion buffer) were drawn through the flow chamber at various flow rates (resulting in estimated fluid shear stresses) for 3 minutes, as detailed in “Materials and methods.” Studies were initiated at 2.0 dynes/cm2 and subsequently the flow rate reduced to 1.5 dynes/cm2, 1.0 dynes/cm2, 0.7 dynes/cm2, and finally to 0.5 dynes/cm2 every 3 minutes. PrMC adhesion was determined at the end of each flow rate and reflects accumulation of adherent and rolling over time during the range of shear stress examined. The data represent mean ± SD from 2 separate experiments.
