Fig. 3.
mAb inhibition of PrMC adhesion to immobilized adhesion molecules.
PrMCs (5 × 105/mL) were preincubated for 15 minutes at RT with blocking mAbs to α4-integrin (HP2/1) or PSGL-1 (KLP-1) or media alone and drawn across coverslips containing saturating concentrations of immobilized P-selectin, E-selectin, or VCAM-1 that had been incubated with media containing saturating concentrations of appropriate blocking or control mAb, or media alone. PrMC adhesion was assessed as described in “Materials and methods.” Data are mean ± SD from 3 separate experiments. The asterisk indicates a value statistically significant from media alone for each molecule tested (P < .05 unpaired ttest).
