Fig. 2.
H-ferritin increases IL-10 production from adherent PBLs by effects on adherent cells.
(A) A total of 1 × 105 PBLs and nonadherent PBLs were pretreated for 24 hours with 1 μg/mL H-ferritin and stimulated with 50 ng anti-CD3 for 3 days. Inhibition of proliferation of nonadherent PBLs was seen only when mixed with 5 × 103 or 2 × 104 adherent cells that had been exposed to 1 μg/mL H-ferritin but not L-ferritin. (B) A total of 1 × 105 PBLs and nonadherent PBLs pretreated for 24 hours with 1 μg/mL H-ferritin and stimulated with 50 ng anti-CD3 for 24 hours. IL-10 production was increased only when mixed with 5 × 103 or 2 × 104 adherent cells treated with H-ferritin but not L-ferritin (1 μg/mL).
