Fig. 3.
H-ferritin increases INF-γ production through a direct effect on nonadherent PBLs and IL-10 production through an indirect effect involving DCs.
(A) Pretreatment of 1 × 105 nonadherent PBLs stimulated with 50 ng anti-CD3 with H-ferritin (1 μg/mL) has a direct effect on IFN-γ production that was further enhanced by exposure to 1 μg/mL H-ferritin–pretreated 1 × 104 DCs (24 hours). (B) Pretreatment of DCs with H-ferritin for 24 hours results in increased production of IL-10 from nonadherent PBLs stimulated with anti-CD3 at 24 hours.
