H-ferritin increases INF-γ production through a direct effect on CD8⁺ T cells and IL-10 production through an indirect effect on CD4⁺ T cells involving DCs.

The CD4 populations were 95% pure and the CD8 populations 94% pure. (A) Addition of 1 × 10⁴ H-ferritin– (1 μg/mL) pretreated DCs to 1 × 10⁵ anti-CD3–stimulated nonadherent CD4 and CD8 subpopulations increased IL-10 production (24 hours) predominantly from CD4 T cells. (B) H-ferritin induces IFN-γ production in nonadherent PBLs CD8 T cells, and this was amplified by DCs pretreated with H-ferritin (1 μg/mL).