Fig. 7.
H-ferritin induces IL-10 production by interaction with the CTLA-4 and PD-1, but not CD28 receptor on T cells and B7-H1 ligand on adherent PBMCs.
The mAbs against B7-H1 (A), PD-1 (B), and CTLA-4 (C) inhibited the induction of IL-10 by H-ferritin. An mAb against CD28 enhanced IL-10 production by H-ferritin (D). An isotype control had no effect on IL-10 production. A total of 2 × 105 PBLs were cultured with 1 μg/mL H- or L-ferritin in a 96-well plate that was prebound with a serial dilution of mAb and 10 μg/mL anti-CD3 for 24 hours. IL-10 was measured by ELISA in triplicate.
