H-ferritin pretreatment of DCs stimulates a different response in T cells to CD40L-pretreated DCs.
(A) Functional ability of monocyte-derived DCs exposed to 1 μg/mL H- or L-ferritin (24 hours) or CD40L (48 hours) to stimulate allo-MLRs. A serial dilution (1 × 104 to 1 cells per well) of DCs was cultured in triplicate with 1 × 105 allogeneic nonadherent PBLs responder cells and cultured for 5 days. (B) Suppression of anti-CD3 lymphocyte proliferation by CD25+ T regulatory cells is enhanced through interaction with H-ferritin–pretreated DCs. CD25+ subpopulations were precultured for 24 hours with H-ferritin–, L-ferritin–, or CD40L– (1 μg/mL) pretreated DCs, harvested, and then treated with mitomycin-C, before addition to anti-CD3–stimulated PBLs.
