Fig. 2.
![Fig. 2. Messenger RNase protection analysis and ligand binding studies on transfected 32D/G-CSF-R cells. / (A) RNase protection analysis using probe P on 10 μg total mRNA from 4 distinct transfected 32D/G-CSF-R clones: 2 32D/G-CSF-R clones transfected with Cb2 exon-1B/exon-2 cDNA and 2 clones with control vector pLNCX. (B) Cb2 receptor density and receptor affinity for CP55940 on 2 Cb2 exon-1B/exon-2 clones and 2 empty vector control clones. Density and affinity were assessed by saturation radioligand receptor binding experiments using [3H]CP55940. Kd is added to the columns.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/8/10.1182_blood.v99.8.2786/5/h80822431002.jpeg?Expires=1735516601&Signature=CPMoGrSCXdQIIh5s3Vw1S-E8cQifM--rGapLG0gb49G1PVZ-YAo01erlHb~TwYph~nMS6q5ayS9ZEnLCNOyjQQbEiZS9FjZC5aOpckrQUoHU9C2KqnST36DI1gmoFkd6ISTxJ8iROWCDsx3M4Bf5mo1gyxDlFHETZyOfYYYW0~3wt8CaVFvlL~V0Wki7Sjx~ZLmJ0yHcvBF1DQaPtts49y3Hsm4uMivRbVmE7MMB5wk2ECzlGeBCUrEspPD4i2f9Qx6plCk3kX9lmtV~faAftwcheLxXbMo2b4SiQ7LIAw~6R1HjaNXtx2~9btZRYA4dxC7iZUdsyB7CpJBHyq2kNA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Messenger RNase protection analysis and ligand binding studies on transfected 32D/G-CSF-R cells.
(A) RNase protection analysis using probe P on 10 μg total mRNA from 4 distinct transfected 32D/G-CSF-R clones: 2 32D/G-CSF-R clones transfected with Cb2 exon-1B/exon-2 cDNA and 2 clones with control vector pLNCX. (B) Cb2 receptor density and receptor affinity for CP55940 on 2 Cb2 exon-1B/exon-2 clones and 2 empty vector control clones. Density and affinity were assessed by saturation radioligand receptor binding experiments using [3H]CP55940. Kd is added to the columns.
