In vitro migration of Cb2-expressing cells following exposure to cannabinoids.
(A) Comparison of the chemoattractive effect of different cannabinoid ligands (1 μM) on Cb2 exon-1B/exon-2–expressing 32D/G-CSF-R cells. Ligands were added to the lower chamber, and cells that migrated to the lower well were counted after 4 hours of incubation. Y-axis shows the percentage of migrated cells from an input of 1 × 105. (B) Effect of different concentrations of 2-AG when added to the lower chamber on the in vitro migration ofCb2-expressing 32D/G-CSF-R cells. Y-axis shows the percentage of migrated cells from an input of 1 × 105. (C) 2-AG titration experiment using the myeloid cell line NFS 78. Y-axis shows the percentage of migrated cells from an input of 2 × 105. (D) Chemoattractive effect of 300 nM 2-AG (▪) or nothing (■) on exon-1B/exon-2 Cb2-expressing cells versus non-Cb2–expressing cells. Y-axis shows percentage of migrated cells from an input of 2 × 105cells. (E) Cb2-expressing 32D/G-CSF-R cells were exposed to medium with or without 300 nM 2-AG added to the lower well; 100 nM Cb1-specific antagonist, SR141716, Cb2-specific antagonist, SR144528, or cells without antagonist as a control were placed on the upper well. Y-axis shows percentage of migration from an input of 1 × 105 cells.
