Vena cava thrombus in wild-type C57BL/6 mice and FVIII^−/−mice reconstituted with rhFVIII.

Inferior vena cava thrombosis was induced by ligation of the vein and endothelial damage resulting from short-term localized compression of the vessel. The surgical procedure was performed in wild-type mice (A-C) and FVIII^−/− mice reconstituted with 7.5 IU rhFVIII (D-F). Four hours following surgery, mice were killed and thrombi analyzed histologically. (A,D) Hematoxylin/eosin-stained microscopic slide of a transverse section. (B,E) MSB stain with red cells, yellow; fresh fibrin, red; mature fibrin, blue/gray. (C,F) Immunostaining of platelets forming dark gray lines of Zahn. Magnification bar: 100 μm.