Pharmacokinetics of rhFVIII and rhFVIII/mAb-LE2E9 complex in FVIII^−/− mice.

FVIII activity was measured in FVIII^−/− mice following administration of rhFVIII in presence or absence of mAb-LE2E9. (A) A total of 7.5 IU rhFVIII (350 IU/kg) was injected intravenously; (B) 100 μL of a mixture containing 7.5 IU rhFVIII and 10 μg mAb-LE2E9 was preincubated for 30 minutes in vitro and administered intravenously; (C) 100 μL containing 7.5 IU rhFVIII was injected intravenously followed after 15 minutes by 100 μL containing 10 μg mAb-LE2E9. FVIII levels were measured in plasma samples taken at indicated periods of time in groups of 3 mice. Results are expressed as mean ± SD.