Primary cultures of endothelial cells express SDF-1.
(A) Immunofluorescence staining of HUVECs grown on gelatin-coated wells. Cells were fixed with 3.5% formaldehyde and stained with a murine monoclonal anti–SDF-1 antibody (IgG1, clone 79018.111). Antibody binding was revealed by FITC-labeled goat antimouse IgG antibodies. Control staining with mouse IgG1 (hybridoma clone 11711.11) and FITC-labeled goat antimouse IgG antibodies was negative (not shown). (B) SDF-1 detected in the supernatant of HUVECs measured by specific ELISA. Cells (3 × 104/mL) plated on Matrigel-coated chamber slides were incubated for the indicated time intervals. Results reflect the means (± SD) of 3 independent HUVEC cultures. Assay sensitivity for SDF-1 was calculated at approximately 10 pg/mL.
