American Society of Hematology

Fig. 2.

$Fig. 2. Western blot analysis of HGF in human neutrophil subcellular fractions. / Human neutrophil subcellular fractions were prepared as described in “Materials and methods”; proteins from each fraction (azurophil and specific granules (gr), membranes and cytosol) were separated by SDS-PAGE in reducing conditions and revealed with antihuman HGF monoclonal antibodies; rh-HGF (a mixture of pro-HGF and mature HGF) was loaded and blotted in parallel. The molecular masses of protein standards are indicated. These data are representative of one typical experiment of 5.$

Western blot analysis of HGF in human neutrophil subcellular fractions.

Human neutrophil subcellular fractions were prepared as described in “Materials and methods”; proteins from each fraction (azurophil and specific granules (gr), membranes and cytosol) were separated by SDS-PAGE in reducing conditions and revealed with antihuman HGF monoclonal antibodies; rh-HGF (a mixture of pro-HGF and mature HGF) was loaded and blotted in parallel. The molecular masses of protein standards are indicated. These data are representative of one typical experiment of 5.

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