Fig. 3.
Effect of degranulating agents on HGF secretion by human PMNs.
Secreted HGF was measured in supernatants of PMNs (107/mL) incubated for 20 minutes at 37°C with 1 μg/mL LPS, 100 ng/mL PMA, 10−8 M, and 10−6 M fMLP. When indicated, PMNs were preincubated with 5 μg/mL cytochalasin B (CYTB). Unstimulated control cells (CTRL) were incubated at 4°C or 37°C in the medium alone. Results are expressed as the mean ± SEM of 3 independent experiments. ND indicates not detected (< 40 pg/mL).
