RT-PCR analysis of HGF mRNA expression by bone marrow cells and mature PMNs.

Total RNA was extracted from bone marrow (BM) cells (previously depleted from their mononuclear cells) and from mature PMNs (PMN). After reverse transcription, PCR was carried out with specific pairs of primers designed for HGF and PBGD. Unstimulated MRC-5 human embryonic lung fibroblasts served as positive controls (+). The figure indicates the size of amplification products relative to molecular weight standards run in parallel (M) and the negative control (−).