Western blot analysis of HGF released after PMN degranulation: colocalization with neutrophil-derived heparan sulfate.

PMNs were stimulated with cytochalasin B and fMLP at 37°C; the supernatant was then subjected to Western blot analysis in reducing conditions and revealed with either antihuman HGF (anti-HGF) or antiheparan sulfate (anti-HS) monoclonal antibodies, as indicated; rh-HGF was loaded and blotted in parallel. These data are representative of one experiment of 3.