Fig. 4.
Differential HLA-DR expression in DCs within cultured PBMCs.
Using 4-color immunostaining, the Lin−HLA-DRhi (R4) and Lin− HLA-DRlo(R5) cells in cultured PBMCs (day 1) were analyzed with respect to the composition of the DC subsets (CD11c+ and CD123+). Isotype control for APC is shown. This pattern is representative of PBMCs cultured for up to 3 days in 3 separate experiments.