Antigen uptake by DCs in cultured PBMCs.

DCs within the PBMC cultures were tested for their ability to take up F-Dx, LY, and F-TT fresh and after culture (A). The amount of antigen uptake was determined by FACS analysis, calculated as the ΔMFI (37°C − 4°C), and expressed as relative fold ΔMFI compared with the baseline value of 1. Hatched bars represent the HLA-DR^hi DC population (R4, Figure 4), and the open bars represent the HLA-DR^lo (R5, Figure 4) population. At least 3 separate experiments were performed. Immature and LPS-matured Mo-DCs, fresh and cultured sorted DCs, and CMRF-44⁺CD14⁻CD19⁻ DCs (n = 3), were tested for their F-Dx uptake capacity (B). Error bars show SEM. (*,P < .05).