Immunohistochemical detection of TF and fibrin in parallel sections of 2 representative myocardial specimens from experimentally induced NR.

Panels A, C, and E demonstrate arteriolar obstruction by atherosclerotic carotid plaque material and adjacent thrombus in a pig injected with carotid scraping (AM1). Panels B, D, and F show arteriolar thrombosis in pig TF2. Red immunoreactivity indicates positive staining with anti-TF (panels A,B) or antifibrin antibodies (panels C,D). Panels E and F represent negative controls stained with isotype antibodies. For comparison, panels G and H depict myocardial vessels from the same animals in uninjected territories stained with antifibrin antibodies. All panels were photographed at the original magnification × 400. The scale bar represents 100 μm.