Fig. 1.
![Fig. 1. CD33 phosphorylation in myeloid cell lines is induced by phorbol esters. / U937 cells or HL-60 cells were labeled with [32P]-orthophosphate for 1 hour and then treated for 12 minutes with either PMA (18 nM), DMSO (1.5%), or control (0.001% DMSO) for 12 minutes. CD33 immunoprecipitates were isolated using My9, blotted onto nitrocellulose, and detected by fluorography.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/9/10.1182_blood.v99.9.3188/6/h80922499001.jpeg?Expires=1735570786&Signature=bAuO8yHdeO45Moih-zKZWXHTF761rEeYYjbU0ik6-gNziAdeI7GeorVyj4XIF7T~XyncY3FBILe8fZq1bMMna7hjDXd1URCBS-Qn7IN5lWNFsBSd-4R2iIIt7Gz6-zQfcEuRYaFaQCRjRIzziRU0AVqIuegBlLdSJDUJguRyM2vRjnu4UB~R~C-M7btIP1jyiDiwGH0dsBQbhnW-W0rblAWEyvu~KI4eKBPy4v5pxnAmalnlQiRuZ~W30Y2~okJg8HeZqjtDC8mdU7A2p8Rj12j1OGQyokQJy8kB3L0bPgzdTDcQuJKZyYfRFv9sCe~7hWhZrC0fRNSVM9kzYzpqdA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CD33 phosphorylation in myeloid cell lines is induced by phorbol esters.
U937 cells or HL-60 cells were labeled with [32P]-orthophosphate for 1 hour and then treated for 12 minutes with either PMA (18 nM), DMSO (1.5%), or control (0.001% DMSO) for 12 minutes. CD33 immunoprecipitates were isolated using My9, blotted onto nitrocellulose, and detected by fluorography.
