HTLV-I–positive cells secrete VEGF and bFGF.

ELISA measurement of secreted VEGF and bFGF. HTLV-I–transformed cells, HTLV-I–negative cells, and endothelial cells were cultured for 72 hours. Levels of VEGF and bFGF in the cell supernatant were measured using a commercial ELISA kit. The amount of VEGF and bFGF proteins in the samples was calculated using a reference curve established from serial dilutions of rhVEGF and rhbFGF proteins. All experiments were performed in triplicate and were repeated a minimum of 3 times.