Fig. 7.
Detection of connexin-43 protein expression.
Total cell extracts from HTLV-I–transformed cells (HuT-102, MT-2, C8166, C91PL) and HTLV-I–negative cells (Jurkat, CEM, Molt4) were analyzed by Western blot using anti-Cx43–specific antibodies. Rat brain and heart extracts were used as positive control. Specificity was demonstrated by competition with Cx43 peptide or omission of anti-Cx43 primary antibody.