Sensitivity and specificity of intracellular Tax protein detection.

(A) Dot plots showing Tax expression in Jurkat (an uninfected T-cell line) cells, MT2 cells (persistently infected with HTLV-1), and PBMCs from a patient with HAM/TSP after 6 hours of in vitro culture. (B) Dot plots showing experiments where different ratios of MT2 to Jurkat cells were mixed, stained for intracellular Tax, and analyzed by flow cytometry. All plots show 20 000 events except for the final plot, which shows 50 000 events. There are 5 cells positive for Tax seen in the final plot.