Role of mPILSAP in VEGF-stimulated proliferation and migration of MSS31 cells.

The mPILSAP is required for VEGF-stimulated proliferation and migration of MSS31 cells. MSS31 cells were preincubated in 0.1% FCS/αMEM for 24 hours and then treated with mPILSAP ODNs for 6 hours. Thereafter, cells were stimulated with 30 ng/mL VEGF for 12 hours. (A) Northern blot analysis showed that AS-ODN specifically eliminated expression of mPILSAP mRNA. AS indicates AS-ODN; S, S-ODN; MIS, MIS-ODN; SCR, SCR-ODN. As a control, 28S RNA from the ethidium bromide–stained gel is also shown. Western blot analysis also showed that AS-ODN specifically down-regulated mPILSAP protein level of VEGF-stimulated cells. (B) Cell proliferation was examined as described in “Materials and methods.” AS-ODN specifically inhibited VEGF-stimulated proliferation of MSS31 cells. Values are means and SDs of triplicate samples. *Significant difference compared with the VEGF-stimulated group (P < .001). (C) Cell migration was examined as described in “Materials and methods.” AS-ODN specifically inhibited VEGF-stimulated migration of MSS31 cells. Values are means and SDs of 4 fields. *Significant difference compared with the value for the corresponding VEGF-stimulated group (P < .001).