Role of mPILSAP in angiogenesis in vivo.

The mPILSAP is required for angiogenesis in vivo. (A) Mouse angiogenesis assay and histological analysis were performed as described in “Materials and methods.” (i) Matrigel alone. (ii) Matrigel containing VEGF and heparin. (iii) Matrigel containing VEGF, heparin, and AS-ODN. (iv) Matrigel containing VEGF, heparin, and S-ODN. (v) Matrigel alone. (vi) Matrigel containing bFGF. (vii) Matrigel containing bFGF and AS-ODN. (viii) Matrigel containing bFGF and S-ODN (×200 magnification). (B) Quantification of angiogenesis was performed as described in “Materials and methods.” Values are means and SDs of 4 animals from each group. *Significant difference compared with the value for the corresponding Matrigel containing VEGF (panel Bi) and bFGF (panel Bii) (P < .001). Hematoxylin-eosin staining was used.