Cell cycle analysis of BaF3-hIAN1 subclones following IL-3 withdrawal.
(A) Western blot analysis of 3 BaF3 subclones that expressed different amounts of the hIAN1 protein. Western blot was performed as described in “Materials and methods.” Lanes 1, 2, and 3 show 3 independent BaF3 subclones that contained various amounts of the hIAN1 protein (BaF3-hIAN1 clone 1, BaF3-hIAN1 clone 2, and BaF3-hIAN1 clone 3). Lane 4 shows the BaF3 parental cell line transfected with an empty vector (BaF3-E), and lane 5 is the hIAN1 protein detected in the Jurkat-ΔCOOH cells. (B) Cell cycle analysis of BaF3-E and BaF3-hIAN1 cells in the absence of IL-3. BaF3-E and BaF3-hIAN1 cells were cultured 14 hours in the absence of IL-3. The cells were then collected, permeabilized, and stained with propidium iodide. The cell cycle profile is expressed as number of cells against DNA content and shows the percentage of cells in each phase (G1, S, G2/M) as indicated.
