Fig. 1.
SCF induced the increase of the intracellular Ca2+concentration.
Ca2+ influx in BAF3/KIT cells induced by SCF or SDF-1α. Cells were loaded with Indo 1-AM for 30 minutes at 37°C and stimulated by SCF at the indicated concentrations or SDF-1α (100 ng/mL). The intracellular Ca2+ concentration was monitored by measuring the relative fluorescence using a spectrofluorometer. Similar results were obtained from 3 independent experiments.