SFKs and PI3K cooperate on SCF signaling for cell migration.

BAF3/KIT cells expressing WT Csk or control vector were untreated or preincubated with 3 μM LY294002 for 30 minutes. Cells were then tested for migration in the presence of 30 ng/mL SCF in the lower chamber. Cells that migrated into the lower chamber were counted and expressed as a percentage for the whole applied cells. The results are shown as the means ± SD of triplicate cultures. The asterisk represents a significant difference from a control value (P < .01). Similar results were obtained from 3 independent experiments.