Time-course of eosinophil and neutrophil apoptosis.

Eosinophils (A) and neutrophils (B) were cultured for the indicated times in medium alone (●), or in the presence of the Fas-activating monoclonal antibody (clone CH-11; 100 ng/mL; ○), IL-5 (10 pM; ■), or GM-CSF (1 ng/mL; ▪) and apoptosis assessed by flow cytometric analysis of PI-stained DNA fragmentation. Data points show the percentage of apoptotic cells and are the mean ± SEM of 3 (A) or 6 (B) independent experiments. P versus control: * < .05, ** < .01, and *** < .005.