Fig. 5.
Eosinophil apoptosis and activation of p36 MBPK is mediated by caspase cleavage.
Cells were pretreated for 2 hours in the absence and presence of 20 μM to 200 μM of the broad specificity caspase inhibitor, z-Asp-CH2-DCB, and then cultured for a further 24 hours. The percentage apoptotic cells (mean ± SEM of 3 independent determinations) was then enumerated by PI-labeling of DNA fragmentation (A). Kinase activity was measured by “in gel” renaturation assays using MBP as a substrate. A representative autoradiograph obtained from 3 independent experiments is shown in B. C indicates control cells at 0 hours. P versus control: * < .05 and *** < .005.
