Collagen- or Cvx-induced platelet aggregation is inhibited by pretreatment with MβCD.

(A, B) Platelets were pre-incubated with the indicated concentration (0, 5, 10, or 20 mM) of MβCD or 20 mM MβCD preloaded with cholesterol (20 + chol) for 30 minutes, washed once, then resuspended in HEPES buffer. Aggregation and ATP release were monitored using lumi-aggregometer on the stimulation of collagen (Col; 5 or 10 μg/mL), Cvx (10 or 20 ng/mL), or PMA (100 nM).