Fig. 3.
Flow cytometry analysis of platelets treated with or without MβCD for surface GPVI and the integrins αIIbβ3 and α2.
Platelets were incubated with 0 (−) or 20 mM (+)MβCD for 30 minutes, washed once, and resuspended in HEPES buffer. (A) Cells were stained with F(ab′)2 fragments of human anti-GPVI IgG or those of control IgG followed by FITC-labeled antihuman IgG antibody and were analyzed by flow cytometry. (B) Cells were stained with anti-αIIbβ3 antibody (P2), anti-α2 antibody (Gi9), or mouse IgG1 as a negative control followed by FITC-labeled antimouse IgG antibody and were analyzed by flow cytometry.
