GPVI–FcRγ downstream signaling is inhibited by MβCD treatment.

Platelets pretreated with (+) or without (−) 20 mM MβCD were unstimulated or stimulated with 50 ng/mL Cvx or 50 μg/mL collagen (Col) for 30 seconds and were lysed in Triton X-100 lysis buffer. Immunoprecipitates (IP) were obtained from the lysates, resolved on SDS-PAGE, and immunoblotted (WB) with each specified antibody. Blots were also reprobed with antiphosphotyrosine antibody (αp-Tyr).