American Society of Hematology

Fig. 5.

$Fig. 5. Localization of the GPVI–FcRγ complex in the low-density sucrose-gradient fractions and effect of MβCD. / Platelets were preincubated without (i,iii) or with (ii, iv) 20 mM MβCD for 30 minutes, washed once, unstimulated (i, ii) or stimulated (iii, iv) with 20 ng/mL Cvx for 30 seconds, and lysed in Triton X-100–containing buffer. The whole lysate was separated into 12 fractions (1-12) by sucrose density (5%-40%) ultracentrifugation. An equal volume of gradient fractions was separated by SDS-PAGE and blotted (WB) with anti-GPVI or anti-FcRγ antibody.$

Localization of the GPVI–FcRγ complex in the low-density sucrose-gradient fractions and effect of MβCD.

Platelets were preincubated without (i,iii) or with (ii, iv) 20 mM MβCD for 30 minutes, washed once, unstimulated (i, ii) or stimulated (iii, iv) with 20 ng/mL Cvx for 30 seconds, and lysed in Triton X-100–containing buffer. The whole lysate was separated into 12 fractions (1-12) by sucrose density (5%-40%) ultracentrifugation. An equal volume of gradient fractions was separated by SDS-PAGE and blotted (WB) with anti-GPVI or anti-FcRγ antibody.

This Feature Is Available To Subscribers Only