American Society of Hematology

Fig. 6.

$Fig. 6. Comparison of the localization of GPVI with that of other membrane proteins within the sucrose density gradients. / Platelets, whose surface membrane proteins were biotinylated, were preincubated without (A) or with (B) 20 mM MβCD for 30 minutes, washed once, and lysed in Triton X-100–containing buffer. Whole lysate was separated into 12 fractions (1-12) by sucrose density (5%-40%) ultracentrifugation. An equal volume of gradient fractions was separated by SDS-PAGE and was blotted with biotinylated anti-GPVI antibody using the streptavidin–HRP/ECL system. Arrow indicates the position of GPVI.$

Comparison of the localization of GPVI with that of other membrane proteins within the sucrose density gradients.

Platelets, whose surface membrane proteins were biotinylated, were preincubated without (A) or with (B) 20 mM MβCD for 30 minutes, washed once, and lysed in Triton X-100–containing buffer. Whole lysate was separated into 12 fractions (1-12) by sucrose density (5%-40%) ultracentrifugation. An equal volume of gradient fractions was separated by SDS-PAGE and was blotted with biotinylated anti-GPVI antibody using the streptavidin–HRP/ECL system. Arrow indicates the position of GPVI.

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