American Society of Hematology

Fig. 7.

$Fig. 7. Sucrose density-gradient fractionation of raft- and nonraft-associated molecules in platelets. / Platelets preincubated without (i, iii) or with (ii, iv) 20 mM MβCD were unstimulated (i, ii) or stimulated (iii, iv) with 20 ng/mL Cvx for 30 seconds, lysed in Triton X-100–containing buffer, and separated into 12 sucrose density fractions (1-12) as described in the legend to Figure 5. An equal volume of gradient fractions was separated by SDS-PAGE and blotted (WB) with each specified antibody.$

Sucrose density-gradient fractionation of raft- and nonraft-associated molecules in platelets.

Platelets preincubated without (i, iii) or with (ii, iv) 20 mM MβCD were unstimulated (i, ii) or stimulated (iii, iv) with 20 ng/mL Cvx for 30 seconds, lysed in Triton X-100–containing buffer, and separated into 12 sucrose density fractions (1-12) as described in the legend to Figure 5. An equal volume of gradient fractions was separated by SDS-PAGE and blotted (WB) with each specified antibody.

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