Specific detection of the T379M substitution by PCR-SSP.

A PCR-SSP was devised that amplified a specific 140-bp product from the aberrant RHD exon 8 in all DAU alleles tested (lane 1: DAU-0; lane 2: DAU-1; lane 3:DAU-2; lane 4: DAU-3; lane 5: DAU-4). In a normal D-positive sample (lane 6), only the 434-bp control product deriving from the human growth hormone gene is amplified.