Fig. 1.
MFGS-gc vector.
IL2RG cDNA was inserted into NcoI andBamHI sites of MFGS, derived from Moloney murine leukemia virus. In addition to packaging and splice sites, 3 nucleotide substitutions have been engineered at positions 1256, 1273, and 1478 of the plasmid to produce termination codons (S) in all reading frames.