Fig. 2.
Fig. 2. Detection of provirus versus genomic DNA in transduced patient cell lines. / (A) PCR using primers flanking IL2RG intron 3 coamplified genomic and provirus DNA in a single PCR reaction to estimate provirus equivalents per genome. Pr indicates producer line 293SPA MFGS-gc; Hu, human genomic DNA. Representative transductions of cell lines from patients 2 and 3 with amphotropic (A)– GALV (G)–, and RD114 (RD)–enveloped retrovirus. (B) Southern blot analysis ofSstI–digested DNA probed with IL2RG cDNA to detect both genomic and provirus DNA.

Detection of provirus versus genomic DNA in transduced patient cell lines.

(A) PCR using primers flanking IL2RG intron 3 coamplified genomic and provirus DNA in a single PCR reaction to estimate provirus equivalents per genome. Pr indicates producer line 293SPA MFGS-gc; Hu, human genomic DNA. Representative transductions of cell lines from patients 2 and 3 with amphotropic (A)– GALV (G)–, and RD114 (RD)–enveloped retrovirus. (B) Southern blot analysis ofSstI–digested DNA probed with IL2RG cDNA to detect both genomic and provirus DNA.

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