Fig. 5.
Fig. 5. Flow cytometric analysis of human cells from chimeric sheep. / Top panels, cells from a sheep receiving control peripheral human CD34+ cells; center panels, a sheep receiving untransduced XSCID CD34+ cells; bottom panels, a sheep receiving XSCID CD34+ cells transduced with RD114 MFGS-gc. Horizontal bars, positive γc gates based on less than 5% background γc expression of sheep cells (CD45−CD3−CD19− or CD45−CD11b−CD15−). For myeloid staining, untransplanted sheep were used as the negative control for setting gates.

Flow cytometric analysis of human cells from chimeric sheep.

Top panels, cells from a sheep receiving control peripheral human CD34+ cells; center panels, a sheep receiving untransduced XSCID CD34+ cells; bottom panels, a sheep receiving XSCID CD34+ cells transduced with RD114 MFGS-gc. Horizontal bars, positive γc gates based on less than 5% background γc expression of sheep cells (CD45−CD3−CD19− or CD45−CD11b−CD15−). For myeloid staining, untransplanted sheep were used as the negative control for setting gates.

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