Fig. 2.
Platelet concentrations and secretory rates of alpha granule components are unaffected in C3H/HeSnJ ashen mice.
(A) Platelets (30 μg protein) of normal C3H/HeSnJ and C3H/HeSnJash/ash mice were incubated in the absence and presence of thrombin for 3 minutes and immediately separated into pellet (p) and supernatant (s) fractions by centrifugation. All fractions were immunoblotted with specific antibody to fibrinogen. (B) Platelets (30 μg protein) were immunoblotted with specific antibody to platelet factor 4.