SU11652, SU11654, and SU11655 inhibit SCF-dependent and SCF-independent phosphorylation of Kit.
Cells (20 × 106 C57) were serum starved for 2 hours, then cultured in complete medium in the absence or presence of SU11652, SU11654, or SU11655 at the indicated concentrations. Cells were collected 2 hours after drug treatment, then incubated with either no rmSCF or rmSCF at 100 ng/mL for 15 minutes at room temperature. 20 × 106 BR, C2, or P815 cells were serum starved for 2 hours, then cultured in complete medium or in SU11652, SU11654, or SU11655 at the indicated concentrations. Cells were collected and lysed, Kit was immunoprecipitated from the lysate, and SDS-PAGE was performed. After transfer to PVDF membrane, the blot was probed for phosphotyrosine, stripped, and reprobed for Kit.
