Dual FISH analysis of CD34⁺CD38⁻ cells from an MDS case with both del(5q) and trisomy 8.

Purified BM CD34⁺CD38⁻ cells from a healthy donor (A) and an MDS case with both del(5q) and trisomy 8 (B) were subjected to dual FISH analysis. The nuclei of cells with normal chromosome 5 and 8 status display 2 orange (5q) and 2 green (8) signals (all 3 cells in panel A). In panel B all 4 nuclei had del(5q) and 3 of these displayed +8 in addition. Original magnification approximately × 1000.