Distinct expression pattern of chemokine receptors among NKT cell subsets.

(A) Chemokine receptor expression by CD4, CD8, and DN NKT subsets (n = 4). (B) Dot plots of chemokine receptor expression by NKT cell subsets with percentages positive. (C) Relative frequencies of CD4, CD8, and DN NKT cell subsets in the Vα24⁺Vβ11⁺ NKT cell population (n = 11). (D) Differential cytokine production capacities of NKT cell subsets (n = 5). Isolated Vβ11⁺ NKT cells were stained with antibodies to Vα24, CD4, and CD8 and activated with phorbol myristate acetate and ionomycin in the presence of monensin to block internalization of surface antigens followed by intracellular cytokine analyses. Bars indicate averages of results from 4 (A), 11 (C), or 5 (D) donors. *Significant differences from the CD4 NKT subset (P < .05 [A,D]). **Significant difference from CD4 or CD8 subset (P < .02 [C]).